The Occurrence of Sulfated Salicinoids in Poplar and Their Formation by Sulfotransferase1.
Identifieur interne : 000082 ( Main/Exploration ); précédent : 000081; suivant : 000083The Occurrence of Sulfated Salicinoids in Poplar and Their Formation by Sulfotransferase1.
Auteurs : Nathalie D. Lackus [Allemagne] ; Andrea Müller [Allemagne] ; Tabea D U. Kröber [Allemagne] ; Michael Reichelt [Allemagne] ; Axel Schmidt [Allemagne] ; Yoko Nakamura [Allemagne] ; Christian Paetz [Allemagne] ; Katrin Luck [Allemagne] ; Richard L. Lindroth [États-Unis] ; C Peter Constabel [Canada] ; Sybille B. Unsicker [Allemagne] ; Jonathan Gershenzon [Allemagne] ; Tobias G. Köllner [Allemagne]Source :
- Plant physiology [ 1532-2548 ] ; 2020.
Abstract
Salicinoids form a specific class of phenolic glycosides characteristic of the Salicaceae. Although salicinoids accumulate in large amounts and have been shown to be involved in plant defense, their biosynthesis is unclear. We identified two sulfated salicinoids, salicin-7-sulfate and salirepin-7-sulfate, in black cottonwood (Populus trichocarpa). Both compounds accumulated in high amounts in above-ground tissues including leaves, petioles, and stems, but were also found at lower concentrations in roots. A survey of salicin-7-sulfate and salirepin-7-sulfate in a subset of poplar (Populus sp.) and willow (Salix sp.) species revealed a broader distribution within the Salicaceae. To elucidate the formation of these compounds, we studied the sulfotransferase (SOT) gene family in Ptrichocarpa (PtSOT). One of the identified genes, PtSOT1, was shown to encode an enzyme able to convert salicin and salirepin into salicin-7-sulfate and salirepin-7-sulfate, respectively. The expression of PtSOT1 in different organs of Ptrichocarpa matched the accumulation of sulfated salicinoids in planta. Moreover, RNA interference-mediated knockdown of SOT1 in gray poplar (Populus × canescens) resulted in decreased levels of sulfated salicinoids in comparison to wild-type plants, indicating that SOT1 is responsible for their formation in planta. The presence of a nonfunctional SOT1 allele in black poplar (Populus nigra) was shown to correlate with the absence of salicin-7-sulfate and salirepin-7-sulfate in this species. Food choice experiments with leaves from wild-type and SOT1 knockdown trees suggest that sulfated salicinoids do not affect the feeding preference of the generalist caterpillar Lymantria dispar A potential role of the sulfated salicinoids in sulfur storage and homeostasis is discussed.
DOI: 10.1104/pp.19.01447
PubMed: 32098786
PubMed Central: PMC7210634
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Lindroth</name><affiliation wicri:level="2"><nlm:affiliation>Department of Entomology, University of Wisconsin-Madison, Madison, Wisconsin 53706.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Wisconsin</region></placeName><wicri:cityArea>Department of Entomology, University of Wisconsin-Madison, Madison</wicri:cityArea></affiliation></author><author><name sortKey="Constabel, C Peter" sort="Constabel, C Peter" uniqKey="Constabel C" first="C Peter" last="Constabel">C Peter Constabel</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Forest Biology, Department of Biology, University of Victoria, Victoria, British Columbia V8W 3N5, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Centre for Forest Biology, Department of Biology, University of Victoria, Victoria, British Columbia V8W 3N5</wicri:regionArea><wicri:noRegion>British Columbia V8W 3N5</wicri:noRegion></affiliation></author><author><name sortKey="Unsicker, Sybille B" sort="Unsicker, Sybille B" uniqKey="Unsicker S" first="Sybille B" last="Unsicker">Sybille B. Unsicker</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena</wicri:regionArea><wicri:noRegion>07745 Jena</wicri:noRegion><wicri:noRegion>07745 Jena</wicri:noRegion><wicri:noRegion>07745 Jena</wicri:noRegion></affiliation></author><author><name sortKey="Gershenzon, Jonathan" sort="Gershenzon, Jonathan" uniqKey="Gershenzon J" first="Jonathan" last="Gershenzon">Jonathan Gershenzon</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena</wicri:regionArea><wicri:noRegion>07745 Jena</wicri:noRegion><wicri:noRegion>07745 Jena</wicri:noRegion><wicri:noRegion>07745 Jena</wicri:noRegion></affiliation></author><author><name sortKey="Kollner, Tobias G" sort="Kollner, Tobias G" uniqKey="Kollner T" first="Tobias G" last="Köllner">Tobias G. Köllner</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany koellner@ice.mpg.de.</nlm:affiliation><country wicri:rule="url">Allemagne</country><wicri:regionArea>Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena</wicri:regionArea><wicri:noRegion>07745 Jena</wicri:noRegion><wicri:noRegion>07745 Jena</wicri:noRegion><wicri:noRegion>07745 Jena</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32098786</idno><idno type="pmid">32098786</idno><idno type="doi">10.1104/pp.19.01447</idno><idno type="pmc">PMC7210634</idno><idno type="wicri:Area/Main/Corpus">000432</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000432</idno><idno type="wicri:Area/Main/Curation">000432</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000432</idno><idno type="wicri:Area/Main/Exploration">000432</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The Occurrence of Sulfated Salicinoids in Poplar and Their Formation by Sulfotransferase1.</title><author><name sortKey="Lackus, Nathalie D" sort="Lackus, Nathalie D" uniqKey="Lackus N" first="Nathalie D" last="Lackus">Nathalie D. Lackus</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena</wicri:regionArea><wicri:noRegion>07745 Jena</wicri:noRegion><wicri:noRegion>07745 Jena</wicri:noRegion><wicri:noRegion>07745 Jena</wicri:noRegion></affiliation></author><author><name sortKey="Muller, Andrea" sort="Muller, Andrea" uniqKey="Muller A" first="Andrea" last="Müller">Andrea Müller</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena</wicri:regionArea><wicri:noRegion>07745 Jena</wicri:noRegion><wicri:noRegion>07745 Jena</wicri:noRegion><wicri:noRegion>07745 Jena</wicri:noRegion></affiliation></author><author><name sortKey="Krober, Tabea D U" sort="Krober, Tabea D U" uniqKey="Krober T" first="Tabea D U" last="Kröber">Tabea D U. Kröber</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena</wicri:regionArea><wicri:noRegion>07745 Jena</wicri:noRegion><wicri:noRegion>07745 Jena</wicri:noRegion><wicri:noRegion>07745 Jena</wicri:noRegion></affiliation></author><author><name sortKey="Reichelt, Michael" sort="Reichelt, Michael" uniqKey="Reichelt M" first="Michael" last="Reichelt">Michael Reichelt</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena</wicri:regionArea><wicri:noRegion>07745 Jena</wicri:noRegion><wicri:noRegion>07745 Jena</wicri:noRegion><wicri:noRegion>07745 Jena</wicri:noRegion></affiliation></author><author><name sortKey="Schmidt, Axel" sort="Schmidt, Axel" uniqKey="Schmidt A" first="Axel" last="Schmidt">Axel Schmidt</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena</wicri:regionArea><wicri:noRegion>07745 Jena</wicri:noRegion><wicri:noRegion>07745 Jena</wicri:noRegion><wicri:noRegion>07745 Jena</wicri:noRegion></affiliation></author><author><name sortKey="Nakamura, Yoko" sort="Nakamura, Yoko" uniqKey="Nakamura Y" first="Yoko" last="Nakamura">Yoko Nakamura</name><affiliation wicri:level="1"><nlm:affiliation>Nuclear Magnetic Resonance Department, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Nuclear Magnetic Resonance Department, Max Planck Institute for Chemical Ecology, 07745 Jena</wicri:regionArea><wicri:noRegion>07745 Jena</wicri:noRegion><wicri:noRegion>07745 Jena</wicri:noRegion><wicri:noRegion>07745 Jena</wicri:noRegion></affiliation></author><author><name sortKey="Paetz, Christian" sort="Paetz, Christian" uniqKey="Paetz C" first="Christian" last="Paetz">Christian Paetz</name><affiliation wicri:level="1"><nlm:affiliation>Nuclear Magnetic Resonance Department, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Nuclear Magnetic Resonance Department, Max Planck Institute for Chemical Ecology, 07745 Jena</wicri:regionArea><wicri:noRegion>07745 Jena</wicri:noRegion><wicri:noRegion>07745 Jena</wicri:noRegion><wicri:noRegion>07745 Jena</wicri:noRegion></affiliation></author><author><name sortKey="Luck, Katrin" sort="Luck, Katrin" uniqKey="Luck K" first="Katrin" last="Luck">Katrin Luck</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena</wicri:regionArea><wicri:noRegion>07745 Jena</wicri:noRegion><wicri:noRegion>07745 Jena</wicri:noRegion><wicri:noRegion>07745 Jena</wicri:noRegion></affiliation></author><author><name sortKey="Lindroth, Richard L" sort="Lindroth, Richard L" uniqKey="Lindroth R" first="Richard L" last="Lindroth">Richard L. Lindroth</name><affiliation wicri:level="2"><nlm:affiliation>Department of Entomology, University of Wisconsin-Madison, Madison, Wisconsin 53706.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Wisconsin</region></placeName><wicri:cityArea>Department of Entomology, University of Wisconsin-Madison, Madison</wicri:cityArea></affiliation></author><author><name sortKey="Constabel, C Peter" sort="Constabel, C Peter" uniqKey="Constabel C" first="C Peter" last="Constabel">C Peter Constabel</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Forest Biology, Department of Biology, University of Victoria, Victoria, British Columbia V8W 3N5, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Centre for Forest Biology, Department of Biology, University of Victoria, Victoria, British Columbia V8W 3N5</wicri:regionArea><wicri:noRegion>British Columbia V8W 3N5</wicri:noRegion></affiliation></author><author><name sortKey="Unsicker, Sybille B" sort="Unsicker, Sybille B" uniqKey="Unsicker S" first="Sybille B" last="Unsicker">Sybille B. Unsicker</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena</wicri:regionArea><wicri:noRegion>07745 Jena</wicri:noRegion><wicri:noRegion>07745 Jena</wicri:noRegion><wicri:noRegion>07745 Jena</wicri:noRegion></affiliation></author><author><name sortKey="Gershenzon, Jonathan" sort="Gershenzon, Jonathan" uniqKey="Gershenzon J" first="Jonathan" last="Gershenzon">Jonathan Gershenzon</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena</wicri:regionArea><wicri:noRegion>07745 Jena</wicri:noRegion><wicri:noRegion>07745 Jena</wicri:noRegion><wicri:noRegion>07745 Jena</wicri:noRegion></affiliation></author><author><name sortKey="Kollner, Tobias G" sort="Kollner, Tobias G" uniqKey="Kollner T" first="Tobias G" last="Köllner">Tobias G. Köllner</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany koellner@ice.mpg.de.</nlm:affiliation><country wicri:rule="url">Allemagne</country><wicri:regionArea>Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena</wicri:regionArea><wicri:noRegion>07745 Jena</wicri:noRegion><wicri:noRegion>07745 Jena</wicri:noRegion><wicri:noRegion>07745 Jena</wicri:noRegion></affiliation></author></analytic><series><title level="j">Plant physiology</title><idno type="eISSN">1532-2548</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Salicinoids form a specific class of phenolic glycosides characteristic of the Salicaceae. Although salicinoids accumulate in large amounts and have been shown to be involved in plant defense, their biosynthesis is unclear. We identified two sulfated salicinoids, salicin-7-sulfate and salirepin-7-sulfate, in black cottonwood (<i>Populus trichocarpa</i>). Both compounds accumulated in high amounts in above-ground tissues including leaves, petioles, and stems, but were also found at lower concentrations in roots. A survey of salicin-7-sulfate and salirepin-7-sulfate in a subset of poplar (<i>Populus</i> sp.) and willow (<i>Salix</i> sp.) species revealed a broader distribution within the Salicaceae. To elucidate the formation of these compounds, we studied the sulfotransferase (<i>SOT</i>) gene family in <i>P</i><i>trichocarpa</i> (<i>PtSOT</i>). One of the identified genes, <i>PtSOT1</i>, was shown to encode an enzyme able to convert salicin and salirepin into salicin-7-sulfate and salirepin-7-sulfate, respectively. The expression of <i>PtSOT1</i> in different organs of <i>P</i><i>trichocarpa</i> matched the accumulation of sulfated salicinoids in planta. Moreover, RNA interference-mediated knockdown of <i>SOT1</i> in gray poplar (<i>Populus</i> × <i>canescens</i>) resulted in decreased levels of sulfated salicinoids in comparison to wild-type plants, indicating that SOT1 is responsible for their formation in planta. The presence of a nonfunctional <i>SOT1</i> allele in black poplar (<i>Populus nigra</i>) was shown to correlate with the absence of salicin-7-sulfate and salirepin-7-sulfate in this species. Food choice experiments with leaves from wild-type and <i>SOT1</i> knockdown trees suggest that sulfated salicinoids do not affect the feeding preference of the generalist caterpillar <i>Lymantria dispar</i> A potential role of the sulfated salicinoids in sulfur storage and homeostasis is discussed.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32098786</PMID><DateRevised><Year>2020</Year><Month>07</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1532-2548</ISSN><JournalIssue CitedMedium="Internet"><Volume>183</Volume><Issue>1</Issue><PubDate><Year>2020</Year><Month>05</Month></PubDate></JournalIssue><Title>Plant physiology</Title><ISOAbbreviation>Plant Physiol</ISOAbbreviation></Journal><ArticleTitle>The Occurrence of Sulfated Salicinoids in Poplar and Their Formation by Sulfotransferase1.</ArticleTitle><Pagination><MedlinePgn>137-151</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1104/pp.19.01447</ELocationID><Abstract><AbstractText>Salicinoids form a specific class of phenolic glycosides characteristic of the Salicaceae. Although salicinoids accumulate in large amounts and have been shown to be involved in plant defense, their biosynthesis is unclear. We identified two sulfated salicinoids, salicin-7-sulfate and salirepin-7-sulfate, in black cottonwood (<i>Populus trichocarpa</i>). Both compounds accumulated in high amounts in above-ground tissues including leaves, petioles, and stems, but were also found at lower concentrations in roots. A survey of salicin-7-sulfate and salirepin-7-sulfate in a subset of poplar (<i>Populus</i> sp.) and willow (<i>Salix</i> sp.) species revealed a broader distribution within the Salicaceae. To elucidate the formation of these compounds, we studied the sulfotransferase (<i>SOT</i>) gene family in <i>P</i><i>trichocarpa</i> (<i>PtSOT</i>). One of the identified genes, <i>PtSOT1</i>, was shown to encode an enzyme able to convert salicin and salirepin into salicin-7-sulfate and salirepin-7-sulfate, respectively. The expression of <i>PtSOT1</i> in different organs of <i>P</i><i>trichocarpa</i> matched the accumulation of sulfated salicinoids in planta. Moreover, RNA interference-mediated knockdown of <i>SOT1</i> in gray poplar (<i>Populus</i> × <i>canescens</i>) resulted in decreased levels of sulfated salicinoids in comparison to wild-type plants, indicating that SOT1 is responsible for their formation in planta. The presence of a nonfunctional <i>SOT1</i> allele in black poplar (<i>Populus nigra</i>) was shown to correlate with the absence of salicin-7-sulfate and salirepin-7-sulfate in this species. Food choice experiments with leaves from wild-type and <i>SOT1</i> knockdown trees suggest that sulfated salicinoids do not affect the feeding preference of the generalist caterpillar <i>Lymantria dispar</i> A potential role of the sulfated salicinoids in sulfur storage and homeostasis is discussed.</AbstractText><CopyrightInformation>© 2020 American Society of Plant Biologists. All Rights Reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lackus</LastName><ForeName>Nathalie D</ForeName><Initials>ND</Initials><Identifier Source="ORCID">0000-0002-0419-8937</Identifier><AffiliationInfo><Affiliation>Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Müller</LastName><ForeName>Andrea</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kröber</LastName><ForeName>Tabea D U</ForeName><Initials>TDU</Initials><Identifier Source="ORCID">0000-0002-5566-6200</Identifier><AffiliationInfo><Affiliation>Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Reichelt</LastName><ForeName>Michael</ForeName><Initials>M</Initials><Identifier Source="ORCID">0000-0002-6691-6500</Identifier><AffiliationInfo><Affiliation>Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schmidt</LastName><ForeName>Axel</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nakamura</LastName><ForeName>Yoko</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Nuclear Magnetic Resonance Department, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Paetz</LastName><ForeName>Christian</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Nuclear Magnetic Resonance Department, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Luck</LastName><ForeName>Katrin</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lindroth</LastName><ForeName>Richard L</ForeName><Initials>RL</Initials><Identifier Source="ORCID">0000-0003-4587-7255</Identifier><AffiliationInfo><Affiliation>Department of Entomology, University of Wisconsin-Madison, Madison, Wisconsin 53706.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Constabel</LastName><ForeName>C Peter</ForeName><Initials>CP</Initials><Identifier Source="ORCID">0000-0002-7627-7597</Identifier><AffiliationInfo><Affiliation>Centre for Forest Biology, Department of Biology, University of Victoria, Victoria, British Columbia V8W 3N5, 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